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Judy, Your story is an indictment of our medical system. If one of those doctors had given you a B12 test earlier on, think of the misery and ill health you might have been spared. And all that expense spared as well.

It’s probable you’re still not getting optimal treatment. In some people, the serum can show an adequate level of B12, but the CNS can still be grossly deficient in B12. Your symptoms suggest you have subacute combined degeneration (SCD). Nasal sprays are not an adequate treatment for SCD.

Freddd (see his prior posts) has made a remarkable recovery from his SCD based on a protocol of his own devising. It’s a very long road, but you too might be able to experience some recovery. Greg’s company, Mentor Consulting, makes a transdermal product called Red B12, that I think is a much better treatment for B12 deficiency diseases. He gave his email previously: Black Shirt Dress 10 / BLACK I Saw It First yUpzTah0
. Some people have had a recovery previously thought impossible via rigorous application of Red B12. It is possible to replete your CNS B12, and sometimes seemingly permanent nerve damage can be reversed.

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Hi Judyj, Thank you for sharing your remarkable story with the group. As Lynn has said it is a remarkable indictment of the general medical system. Every time I read a story like this I am amazed that vitamin B12 deficiency was missed for so long, particularly considering your neuronal symptoms. As Lynn has said it is such an easy thing to at least try early on and the test for deficiency is so cheap and easy. I am sure that you are now well and truly around the literature on VB12 deficiency. I have attempted to add some more information on my web-site http://www.vitaminb12deficiency.net.au . My reading of the literature suggests that it may take months or even years to reverse the neuronal damage, during this time it is essential that you keep your levels up. Once the damage has been done to the neurones, they can recover, but it is very slow. The lipids and myelin basic protein have a very slow turnover time, so these have to be gradually replaced for you to get normal function. In this regard Freddd’s story is relevant as by persisting he gradually got better. Many studies in the literature appear to not take this slow turn over into consideration and finish too early. As Lynn has also eluded to it is important to get the levels in the CNS up again and several studies have shown that this does not happen with either high dose oral or with sprays or sublinguals, or if it does it is very, very slow, which may explain why it took Freddd so long. It is for this reason that we designed the transdermal product that Lynn eluded to. Apart from the treatment, one of the most important things to also identify is why you became deficient, as this also has a massive implication later on. Evidence suggests that if you are severely vitamin B12 deficient you also have trouble with intestinal absorption of most of your water soluble vitamins, so it would be useful for you to have your levels of these checked. So be patient, you are on a long road, but at least you have turned the corner. You will find that this group is very supportive if you have further questions.

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Millennials are the future of the U.S. economy. But when it comes to their future, and the future of the country, they are a deeply pessimistic generation. EY and EIG conducted a new national public opinion survey of 1,200 Millennials to gauge their views on a variety of issues related to the economy at all levels—personal, local, and national—and the challenges they face almost seven years into the recovery from the Great Recession. The results outlined below paint a complex picture of a generation convinced the economy is failing them, one that is willing to work hard to better their lot, and one that is very uncertain about what comes next.

We Asked Millennials About the Economy: Here Are the Top 10 Takeaways

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Millennials are often painted as lazy and entitled, but the stereotype doesn’t hold up upon closer scrutiny. Millennials value education and hard work, and they’re willing to make sacrifices to get ahead. The Great Recession shaped this generation—18% faced unemployment in 2010, two-­thirds have at least one source of long­-term debt, and their wages have fallen over the past five years—and these after effects still reverberate strongly throughout Millennial life. Millennials may perceive their lot as unfair not out of entitlement, but because they feel like they did their part but haven’t seen the expected benefits.

Our survey found:

Millennials invested in human capital and are willing to work hard to get ahead

Coming of age during a historic economic downturn has severely impacted Millenniallife

Stress levels run high for Millennials

88 percent 64 percent 63 percent 30 percent 40 percent one­-third 78 percent 74 percent 79 percent

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Millennials will be the most educated generation in U.S. history, but they are not convinced that higher education will provide them the same leg-­up on the path to prosperity that it guaranteed earlier generations. Their ambivalence stems in part from the inexorable rise in the cost of college, which has changed its value proposition. Instead of opening doors, many Millennials feel that student debt has boxed them in. In fact, between 2004 and 2014, there was an 89 percent increase in the number of student borrowers, and the average balance per borrower grew by 77 percent.

Our survey found:

Millennials feel ambivalent about higher education and question whether the labor market will hold up its end of the bargain

Millennials’ skepticism isn’t unfounded given the risk they’ve taken on

Instead of feeling empowered through education, a significant share of millennials feelboxed in because of student loans

Two-­thirds
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Determination of surface markers CD11b, CD16b, CD64, and CD66 before and after two different differentiation protocols (). Cells differentiated with DMSO or DMSO and IFNγ expressed significantly …

Figure 3
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() PLB-985 cells differentiated with DMSO and IFNγ effectively phagocytize opsonized with human immunoglobulin G (IgG). cells expressing roGFP2-Orp1 associate with the …

We then monitored the oxidation state of roGFP2-Orp1 in E. coli during phagocytosis. Thus, roGFP2-Orp1-expressing E. coli were co-incubated with differentiated neutrophil-like PLB-985 cells and the ratio of fluorescent intensity at 405/488 nm excitation was measured as readout of the probe’s oxidation state in a fluorescence plate reader. The oxidation state of the probe increased, when opsonized E. coli expressing roGFP2-Orp1 were incubated with neutrophil-like cells. The oxidation kinetic showed a gradual increase in the 405/488 nm ratio, as soon as E. coli was incubated with neutrophil-like PLB-985, reaching a plateau after 60 min of incubation and remaining stably oxidized until the end of the measurement (2 hr). In contrast, the probe was not oxidized when bacteria were incubated with medium or undifferentiated cells ( Mens L2285ogan 1b LowTop Sneakers Tommy Hilfiger am3tUMJ
). When E. coli expressing Grx1-roGFP2 was cultured with neutrophil-like PLB-985 cells, the redox response of the probe was highly similar to the response observed with roGFP2-Orp1 ( Figure 4B ), and we essentially observed the same oxidation kinetics with the unfused roGFP2 probe ( Figure 4C ).

Figure 4
Download asset Open asset

cells expressing roGFP2-Orp1 from a plasmid were incubated in a 96-well plate reader. The ratio of the fluorescence intensity at excitation wavelengths of 405 and 488 nm was calculated and …

The oxidation kinetics of the roGFP2-Orp1 probe in E. coli cultured with differentiated PLB-985 cells was not as fast as in an E. coli bacteria suspension that was directly exposed to high concentrations of H 2 O 2 (compare Iridessa Dress in Lavender size L also in MSXLXSXXS Majorelle London S DRu7X
and Figure 4A ). We argued that this could mean that bacteria experience redox stress from the host cells only upon phagocytosis. To examine if phagocytosis indeed plays a role in probe oxidation in the E. coli cytoplasm, we blocked phagocytosis by treating the neutrophil-like cells with Cytochalasin D (CD). Cytochalasin D-pre-treated, differentiated PLB-985 cells did phagocytize opsonized E. coli less effectively than untreated, differentiated PLB-985 cells or cells treated with DMSO (vehicle control) ( Figure 5A ). To verify the effective blocking of phagocytosis, we used opsonized FITC-labeled Zymosan and tested the phagocytic capacity of Cytochalasin D-treated and untreated PLB-985 cells. In this case, we quenched the fluorescence of attached Zymosan particles, giving us a more exact readout of the phagocytic capacity. Zymosan phagocytosis was almost completely blocked in Cytochalasin D-treated neutrophil-like cells when compared to the control or cells pre-incubated with DMSO (vehicle control) ( Figure 5B ). Having established the inhibition of phagocytosis, we tested the redox state of roGFP2-Orp1 in E. coli in the presence of differentiated, Cytochalasin D-treated PLB-985 cells. The 405/488 nm ratio of roGFP2-Orp1 did not change significantly over time ( Figure 5C ). Similarly, oxidation of the Grx1-roGFP2 fusion and unfused roGFP2 probe was phagocytosis-dependent ( Men Watepro Maipo Low Rise Hiking Shoes Merrell i7jx2
) indicating that bacteria are exposed to reactive oxygen species only when internalized.

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